| Time |
Program |
| 09:00 - 09:05 |
Opening Address |
| Kazuo Sutoh (The University of Tokyo) |
| 09:05 - 09:45 |
Plenary Lecture
Chairperson: Kazuo Sutoh |
Ian Gibbons (University of California Berkeley)
NATURAL HISTORY OF THE DYNEIN HEAVY CHAIN GENE FAMILY IN EUKARYOTES |
| Session I. Molecular Structure and Motile Mechanism of Dynein |
| Chairpersons : Michael Koonce, Kazuhiro Oiwa |
| 09:50 - 10:15 |
Stan A. Burgess (University of Leeds)
STRUCTURE AND MECHANISM OF THE DYNEIN MOTOR STUDIED BY ELECTRON MICROSCOPY |
| 10:15 - 10:40 |
Keiko Hirose (National Institute of Advanced Industrial Science and Technology)
STRUCTURAL STUDIES OF DYNEIN BOUND TO MICROTUBULES |
| 10:40 - 10:55 |
Bara Malkova (University of Leeds)
SUBSTRUCTURE OF THE UNDOCKED DYNEIN LINKER REVEALED BY TRUNCATION OF THE C-TERMINAL SEQUENCE |
| 10:55 - 11:15 |
Coffee Break |
| Chairpersons : Stan Burgess, Keiko Hirose |
| 11:15 - 11:40 |
Masahide Kikkawa (The University of Tokyo)
CRYO-EM STUDIES OF HEAD AND STALK OF DYNEINS |
| 11:40 - 12:05 |
Andrew Carter (University of California, San Francisco)
STRUCTURE AND FUNCTIONAL ROLE OF DYNEIN'S MICROTUBULE-BINDING DOMAIN |
| 12:05 - 12:30 |
Michael Koonce (Wadsworth Center)
A COUNTERPOISED SWITCH WITHIN THE DYNEIN STALK DRIVES MICROTUBULE-BINDING AFFINITY |
| 12:30 - 12:45 |
Yusuke Kato (University of Leeds)
STRUCTURE OF THE MICROTUBULE-BINDING DOMAIN OF FLAGELLAR DYNEIN |
| 12:45 - 14:00 |
Lunch |
| Chairpersons : Samara Reck-Peterson, Hitoshi Sakakibara |
| 14:00 - 14:25 |
Takahide Kon (The University of Tokyo)
DISSECTION OF INTRAMOLECULAR COMMUNICATIONS BETWEEN THE CATALYTIC HEAD AND MICROTUBULE-BINDING DOMAINS IN THE DYNEIN HEAVY CHAIN |
| 14:25 - 14:50 |
Tomohiro Shima (The University of Tokyo)
WALKING MECHANISM OF CYTOPLASMIC DYNEIN |
| 14:50 - 15:15 |
Arne Gennerich (Albert Einstein College of Medicine)
TOWARD A UNIFIED WALKING MODEL FOR CYTOPLASMIC DYNEIN |
| 15:15 - 15:40 |
Hideo Higuchi (The University of Tokyo)
WORKING AND FLUCTUATION DISTANCES CONTRIBUTING TO THE STEP SIZE OF SINGLE MOLECULES OF CYTOPLASMIC DYNEIN |
| 15:40 - 16:00 |
Coffee Break |
| 16:00 - 18:30 |
Poster Preview Session |
| Time |
Program |
| Session II. Function and Regulation of Cytoplasmic Dynein |
| Chairpersons : Erika Holzbaur, Takahide Kon |
| 09:00 - 09:25 |
Samara Reck-Peterson (Harvard Medical School)
MECHANISM AND REGULATION OF CYTOPLASMIC DYNEIN MOTOR ACTIVITY |
| 09:25 - 09:50 |
Yoko Yano Toyoshima (The University of Tokyo, Graduate School of Arts and Sciences)
REGURATIOIN AND CORDINATION OF CYTOPLASMIC DYNEIN REVEALED BY IN VITRO STUDY |
| 09:50 - 10:15 |
Shinji Hirotsune (Osaka City University Graduate School of Medicine)
LIS1 AND NUDEL1 COORDINATE THE PLUS-END DIRECTED TRANSPORT OF CYTOPLASMIC DYNEIN |
| 10:15 - 10:40 |
Richard Vallee (Columbia University College of Physicians and Surgeons)
LIS1 AND NUDE CONVERT DYNEIN TO A NOVEL PERSISTENT-FORCE STATE FOR NEURAL STEM CELL MIGRATION |
| 10:40 - 11:00 |
Coffee Break |
| Chairpersons : Richard Vallee, Hideo Higuchi |
| 11:00 - 11:25 |
Erika Holzbaur (University of Pennsylvania School of Medicine)
MOTOR COORDINATION DURING BIDIRECTIONAL VESICLE TRANSPORT ALONG THE AXON |
| 11:25 - 11:40 |
Roop Mallik (Tata Institute of Fundamental Research)
WHY DYNEIN IS DIFFERENT FROM KINESIN: DIRECT OPTICAL TRAP MEASUREMENT OF ENSEMBLE MOTOR FUNCTION ON ENDOSOMES |
| 11:40 - 12:05 |
Trina Schroer (The Johns Hopkins University)
NEW INSIGHTS INTO DYNACTIN STRUCTURE AND FUNCTION |
| 12:05 - 12:20 |
Christian Eckert (Max-Planck-Institute)
STRUCTURAL CHARACTERIZATION OF THE CYTOPLASMIC DYNEIN HEAVY CHAIN FROM HUMAN AND DICTYOSTELIUM DISCOIDEUM |
| 12:20 - 12:35 |
Walid Houry (University of Toronto)
THE HSP110 MOLECULAR CHAPERONE MODULATES DYNEIN ACTIVITY |
| 12:35 - 14:00 |
Lunch |
| Session III. Dynein as the Force Generator for Axonemal Beating |
| Chairpersons : Stephen King, Masahide Kikkawa |
| 14:00 - 14:25 |
Daniela Nicastro (Brandeis University)
CRYO ELECTRON TOMOGRAPHY OF AXONEMAL DYNEIN |
| 14:25 - 14:50 |
Takashi Ishikawa (ETH Zurich)
ELECTRON CRYO-TOMOGRAPHY OF INNER AND OUTER DYNEIN ARMS IN SITU |
| 14:50 - 15:15 |
Toshiki Yagi (Kyoto University)
IDENTIFICATION OF ALL DYNEIN HEAVY CHAIN GENE PRODUCTS IN CHLAMYDOMONAS |
| 15:15 - 15:30 |
Mingyue JIN (Kyushu Institute of Technology)
STRUCTURE OF FLAGELLAR OUTER DYNEIN ARM AND THE STRUCTURAL MECHANISM IN FORMATION OF THE OUTER DYNEIN ARM-MICROTUBULE COMPLEXES |
| 15:30 - 15:50 |
Coffee Break |
| Chairpersons : David Mitchell, Toshiki Yag |
| 15:50 - 16:15 |
Shinji Kamimura (Faculty of Science and Engineering, Chuo University)
X-RAY FIBER DIFFRACTION ANALYSIS OF AXONEME AND MICROTUBULE STURCUTURE USING A NOVEL SHEAR-FLOW FIBER-ALIGNING TECHNIQUE |
| 16:15 - 16:40 |
Chikako Shingyoji (The University of Tokyo)
MECHANISM OF FLAGELLAR OSCILLATION: CONDITIONS FOR THE INDUCTION OF CONTINUOUS BEATING BY EXTERNALLY APPLIED BENDING IN DEMEMBRANATED MOTIIONLESS SEA URCHIN SPERM FLAGELLA AT LOW ATP |
| 16:40 - 17:05 |
Charles Lindemann (Oakland University)
NUCLEOTIDE REGULATION OF DYNEIN CONTROLS THE T-FORCE IN THE FLAGELLAR BEAT CYCLE |
| 17:10 - 17:50 |
Poster Session A <Odd number> |
| 17:50 - 18:30 |
Poster Session B <Even number> |
| Time |
Program |
| Session III. Dynein as the Force Generator for Axonemal Beating |
| Chairpersons : Pietro Lupetti, Chikako Shingyoji |
| 09:00-09:25 |
Pietro Lupetti (University of Siena)
ELECTRON-TOMOGRAPHIC ANALYSIS OF INTRAFLAGELLAR TRANSPORT COMPLEXES IN SITU |
| 09:25-09:50 |
Karl-Ferdinand Lechtreck (University of Massachusetts Medical School)
THE CHLAMYDOMONAS BBSOME IS A CARGO ADAPTOR FOR RETROGRADE IFT |
| 09:50-10:15 | David Mitchell (SUNY Upstate Medical University)
A MULTI-STEP PATHWAY FOR AXONEMAL DYNEIN ASSEMBLY. |
| 10:15-10:40 | Hitoshi Sakakibara (National Institute of Information and Communications Technology)
STRUCTURE, FUNCTION, AND IN VIVO ARRANGEMENT OF CHLAMYDOMONAS AXONEMAL COMPONENTS, INNER-ARM DYNEIN-C, -F, AND RADIAL SPOKE |
| 10:40-11:00 | Coffee Break |
| Session IV. Regulation of Axonemal Dynein |
| Chairpersons : Mary Porter, Shinji Kamimura |
| 11:00-11:25 | Ritsu Kamiya (The University of Tokyo)
REGULATION OF AXONEMAL DYNEINS BY TUBULIN POLYGLUTAMYLATION |
| 11:25-11:50 | Kazuo Inaba (University of Tsukuba)
CAMP AND CA2+-DEPENDENT REGULATION OF AXONEMAL DYNEINS AT ACTIVATION OF SPERM MOTILITY |
| 11:50-12:00 | Group Photo |
| 12:00-14:00 | Lunch |
| Chairpersons : Winfield Sale, Ritsu Kamiya |
| 14:00-14:25 | Stephen King (University of Connecticut Health Center)
THE γ HEAVY CHAIN WITHIN OUTER ARM DYNEIN ACTS AS A KEY REGULATORY NODE FOR CILIARY/FLAGELLAR MOTILITY |
| 14:25-14:50 | Elizabeth F. Smith (Dartmouth College)
MODULATING CILIARY MOTILITY: A ROLE FOR CALMODULIN INTERACTORS ASSOCIATED WITH THE SPOKES AND CENTRAL APPARATUS |
| 14:50-15:15 | Mary Porter (University of Minnesota)
NEW INSIGHTS INTO THE DYNEIN REGULATORY COMPLEX |
| 15:15-15:40 | Winfield S. Sale (Emory University School of Medicine)
REGULATION OF DYNEIN-DRIVEN MICROTUBULE SLIDING BY THE AXONEMAL KINASE CK1 AND THE AXONEMAL PHOSPHATASE PP2A |
| 15:40-15:50 | Closing Remarks |
| Kazuhiro Oiwa (National Institute of Information Communications Technology) |
| 18:00-20:00 | Dinner |
